Generation of conditionally immortalized hematopoietic progenitors
BACKGROUND & KEY OBSERVATIONS
Hox genes are developmentally important transcription factors. Constitutively active Hox-mutants are oncogenes. Estrogen-regulated Hox-genes can be used to block cell differentiation. The combination of regulated Hox-gene and specific growth factor can be used to immortalize specific progenitor cells with neutrophil potential (Hoxb8-SCF (SCF)) or multi-lineage potential (Hoxb8-FL, FLT3L) (see publication below).
Conditional immortalization of an erythrocyte/ megakaryocate progenitor.
Application of Hox-cells in various models, including inflammatory and infectious disease models to study cell differentiation and contribution of specific immune cell populations to immunity.