Browse our lab publications to learn about our contributions to a variety of research areas. PUBLICATIONS Journal articles Review articles Patents Journal Articles Peer-reviewed journal articles 2025 Wu R, Salehi F, Redecke V, Ma Z, Marchetti M, Finkelstein D, Xu P, Cheng Y, Queisser KA, Petrey AC, Field CO, Ahn HS, Poncz M, Weiss MJ, Häcker H. Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential. Nat Commun. 2025 Aug 7;16(1):7283. doi: 10.1038/s41467-025-62668-z.PMID: 40775216 Jawad M, Akhi AT, Wendling R, Redecke V, Häcker H, Gale B. A disposable, passive microfluidic cartridge for point-of-care detection of antibodies in total capillary blood based on hemagglutination and machine-learning assisted interpretation. RSC Adv. 2025 Nov 7;15(51):43322-43333. doi: 10.1039/d5ra05719a. eCollection 2025 Nov 6.PMID: 41209517 2024 Kim AB, Xiao Q, Yan P, Pan Q, Pandey G, Grathwohl S, Gonzales E, Xu I, Cho Y, Haecker H, Epelman S, Diwan A, Lee JM, DeSelm CJ. Chimeric antigen receptor macrophages target and resorb amyloid plaques. JCI Insight. 2024 Feb 6;9(6):e175015. doi: 10.1172/jci.insight.175015.PMID: 38516884 Al-Rabadi LF, Storey A, Larsen CP, Hassen SI, Revelo MP, Singh RS, Lazar-Molnar E, Jain D, Ibrahim A, Darras F, Salam R, Al-Rabadi A, Wikan N, Redecke V, Talwar M, Haecker H, Beck LH Jr, Caza TN. De novo Membranous Nephropathy in Renal Allografts is Associated With Protocadherin FAT1. 2023 Häcker H.. In Vitro Generation of Murine Dendritic Cells from Hoxb8-Immortalized Hematopoietic Progenitors. Methods Mol Biol. 2023;2618:93-107. doi: 10.1007/978-1-0716-2938-3_7.PMID: 36905511 2022 Tawaratsumida K, Redecke V, Wu R, Kuriakose J, Bouchard JJ, Mittag T, Lohman BK, Mishra A, High AA, Häcker H. A phospho-tyrosine-based signaling module using SPOP, CSK, and LYN controls TLR-induced IRF activity. Science Advances 2022 Jul 8;8(27):eabq0084. doi: 10.1126/sciadv.abq0084. Epub 2022 Jul 8. PMID: 35857476 PMCID: PMC9269885. 2021 Redecke V, Tawaratsumida K, Larragoite ET, Williams ESCP, Planelles V, Spivak AM, Hirayama L, Elgort M, Swenson S, Smith R, Worthen B, Zimmerman R, Slev P, Cahoon B, Astill M, Häcker H. A rapid and affordable point of care test for antibodies against SARS-CoV-2 based on hemagglutination and artificial intelligence interpretation. Sci Rep. 2021 Dec 30;11(1):24507. doi: 10.1038/s41598-021-04298-1. PubMed PMID: 34969960; PubMed Central PMCID: PMC8718524. Elhag S, Stremmel C, Zehrer A, Plocke J, Hennel R, Keuper M, Knabe C, Winterhalter J, Gölling V, Tomas L, Weinberger T, Fischer M, Liu L, Wagner F, Lorenz M, Stark K, Häcker H, Schmidt-Supprian M, Völker U, Jastroch M, Lauber K, Straub T, Walzog B, Hammer E, Schulz C. Differences in Cell-Intrinsic Inflammatory Programs of Yolk Sac and Bone Marrow Macrophages. Cells. 2021 Dec 17;10(12). doi: 10.3390/cells10123564. PubMed PMID: 34944072; PubMed Central PMCID: PMC8699930. Xu H, Look T, Prithiviraj S, Lennartz D, Cáceres MD, Götz K, Wanek P, Häcker H, Kramann R, Seré K, Zenke M. CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells. Eur J Immunol. 2021 Nov 26;. doi: 10.1002/eji.202149482. [Epub ahead of print] PubMed PMID: 34826338. 2020 Piperno GM, Naseem A, Silvestrelli G, Amadio R, Caronni N, Cervantes-Luevano KE, Liv N, Klumperman J, Colliva A, Ali H, Graziano F, Benaroch P, Haecker H, Hanna RN, Benvenuti F.(2020) Wiskott-Aldrich syndrome protein restricts cGAS/STING activation by dsDNA immune complexes. JCI Insight. 2020 Sep 3;5(17):e132857. doi: 10.1172/jci.insi ght.132857. Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt M. (2020) Microtubules control cellular shape and coherence in amoeboid migrating cells. J Cell Biol. 2020 Jun ;219(6):e201907154. doi: 10.1083/jcb.201907154. 2019 Kuriakose J, Redecke V, Guy C, Zhou J, Wu R, Ippagunta SK, Tillman H, Walker PD, Vogel P, Häcker H (2019). Patrolling monocytes promote the pathogenesis of early lupus-like glomerulonephritis.LID - 10.1172/JCI125116 [doi]LID - 125116 [pii]. J Clin Invest, 130. 2018 Pollock JA, Sharma N, Ippagunta SK, Redecke V, Häcker H, Katzenellenbogen JA (2018). Triaryl Pyrazole Toll-Like Receptor Signaling Inhibitors: Structure-Activity Relationships Governing Pan- and Selective Signaling Inhibitors. ChemMedChem, 13(20), 2208-2216. Ippagunta SK, Pollock JA, Sharma N, Lin W, Chen T, Tawaratsumida K, High AA, Min J, Chen Y, Guy RK, Redecke V, Katzenellenbogen JA, Häcker H (2018). Identification of Toll-like receptor signaling inhibitors based on selective activation of hierarchically acting signaling proteins.LID - eaaq1077 [pii]LID - 10.1126/scisignal.aaq1077 [doi]. Sci Signal, 11(543). Leithner A, Renkawitz J, De Vries I, Hauschild R, Häcker H, Sixt M (2018). Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration. Eur J Immunol, 48(6), 1074-1077. 2017 Yang CH, Wang Y, Sims M, Cai C, He P, Häcker H, Yue J, Cheng J, Boop FA, Pfeffer LM (2017). MicroRNA203a suppresses glioma tumorigenesis through an ATM-dependent interferon response pathway. Oncotarget, 8(68), 112980-112991. Di Ceglie I, van den Akker GG, Ascone G, Ten Harkel B, Häcker H, van de Loo FA, Koenders MI, van der Kraan PM, de Vries TJ, Vogl T, Roth J, van Lent PL (2017). Genetic modification of ER-Hoxb8 osteoclast precursors using CRISPR/Cas9 as a novel way to allow studies on osteoclast biology. J Leukoc Biol, 101(4), 957-966. Penkert RR, Jones BG, Häcker H, Partridge JF, Hurwitz JL (2017). Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines. Cytokine, 91, 1-5. Grajkowska LT, Ceribelli M, Lau CM, Warren ME, Tiniakou I, Nakandakari Higa S, Bunin A, Häcker H, Mirny LA, Staudt LM, Reizis B (2017). Isoform-Specific Expression and Feedback Regulation of E Protein TCF4 Control Dendritic Cell Lineage Specification. Immunity, 46(1), 65-77. 2016 Ippagunta SK, Gangwar R, Finkelstein D, Vogel P, Pelletier S, Gingras S, Redecke V, Häcker H (2016). Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function. Proc Natl Acad Sci U S A, 113(41), E6162-E6171. Kaewborisuth C, Zanin M, Häcker H, Webby RJ, Lekcharoensuk P (2016). G45R mutation in the nonstructural protein 1 of A/Puerto Rico/8/1934 (H1N1) enhances viral replication independent of dsRNA-binding activity and type I interferon biology. Virol J, 13, 127. Redecke V, Chaturvedi V, Kuriakose J, Häcker H (2016). SHARPIN controls the development of regulatory T cells. Immunology, 148(2), 216-26. 2015 Bunin A, Sisirak V, Ghosh HS, Grajkowska LT, Hou ZE, Miron M, Yang C, Ceribelli M, Uetani N, Chaperot L, Plumas J, Hendriks W, Tremblay ML, Häcker H, Staudt LM, Green PH, Bhagat G, Reizis B (2015). Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells. Immunity, 43(2), 277-88. Wache C, Klein M, Ostergaard C, Angele B, Häcker H, Pfister HW, Pruenster M, Sperandio M, Leanderson T, Roth J, Vogl T, Koedel U (2015). Myeloid-related protein 14 promotes inflammation and injury in meningitis. J Infect Dis, 212(2), 247-57. 2014 Tawaratsumida K, Phan V, Hrincius ER, High AA, Webby R, Redecke V, Häcker H (2014). Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor. J Virol, 88 (16), 9038-48. Schwab L, Goroncy L, Palaniyandi S, Gautam S, Triantafyllopoulou A, Mocsai A, Reichardt W, Karlsson FJ, Radhakrishnan SV, Hanke K, Schmitt-Graeff A, Freudenberg M, von Loewenich FD, Wolf P, Leonhardt F, Baxan N, Pfeifer D, Schmah O, Schonle A, Martin SF, Mertelsmann R, Duyster J, Finke J, Prinz M, Henneke P, Häcker H, Hildebrandt GC, Häcker G, Zeiser R (2014). Neutrophil granulocytes recruited upon translocation of intestinal bacteria enhance graft-versus-host disease via tissue damage. Nat Med, 20(6), 648-54. Klein M, Brouwer MC, Angele B, Geldhoff M, Marquez G, Varona R, Häcker G, Schmetzer H, Häcker H, Hammerschmidt S, van der Ende A, Pfister HW, van de Beek D, Koedel U (2014). Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis. PLoS ONE, 9(4), e93057. 2013 Gautam S, Kirschnek S, Gentle IE, Kopiniok C, Henneke P, Häcker H, Malleret L, Belaaouaj A, Häcker G (2013). Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-beta (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation. Cell Death Differ, 20(8), 1068-79. Redecke V, Wu R, Zhou J, Finkelstein D, Chaturvedi V, High AA, Häcker H (2013). Hematopoietic progenitor cell lines with myeloid and lymphoid potential. Nat Methods, 10(8), 795-803. Hohne C, Wenzel M, Angele B, Hammerschmidt S, Häcker H, Klein M, Bierhaus A, Sperandio M, Pfister HW, Koedel U (2013). High mobility group box 1 prolongs inflammation and worsens disease in pneumococcal meningitis. Brain, 136(Pt 6), 1746-59. LeMessurier KS, Häcker H, Chi L, Tuomanen E, Redecke V (2013). Type I interferon protects against pneumococcal invasive disease by inhibiting bacterial transmigration across the lung. PLoS Pathog, 9(11), e1003727. 2012 Häcker H, Chi L, Rehg JE, Redecke V (2012). NIK prevents the development of hypereosinophilic syndrome-like disease in mice independent of IKKalpha activation. J Immunol, 188(9), 4602-10. 2011 Kirschnek S, Vier J, Gautam S, Frankenberg T, Rangelova S, Eitz-Ferrer P, Grespi F, Ottina E, Villunger A, Häcker H, Häcker G (2011). Molecular analysis of neutrophil spontaneous apoptosis reveals a strong role for the pro-apoptotic BH3-only protein Noxa. Cell Death Differ, 18(11), 1805-14. Zhou J, Wu R, High AA, Slaughter CA, Finkelstein D, Rehg JE, Redecke V, Häcker H (2011). A20-binding inhibitor of NF-kappaB (ABIN1) controls Toll-like receptor-mediated CCAAT/enhancer-binding protein beta activation and protects from inflammatory disease. Proc Natl Acad Sci U S A, 108(44), E998-1006. Stempin CC, Chi L, Giraldo-Vela JP, High AA, Häcker H, Redecke V (2011). The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-kappaB activation. J Biol Chem, 286(43), 37147-57. 2010 Garrison SP, Thornton JA, Häcker H, Webby R, Rehg JE, Parganas E, Zambetti GP, Tuomanen EI (2010). The p53-target gene puma drives neutrophil-mediated protection against lethal bacterial sepsis. PLoS Pathog, 6(12), e1001240. LeMessurier K, Häcker H, Tuomanen E, Redecke V (2010). Inhibition of T cells provides protection against early invasive pneumococcal disease. Infect Immun, 78(12), 5287-94. Woehrl B, Klein M, Rupprecht T, Schmetzer H, Angele B, Häcker H, Häcker G, Pfister HW, Koedel U (2010). CXCL16 contributes to neutrophil recruitment to cerebrospinal fluid in pneumococcal meningitis. J Infect Dis, 202(9), 1389-96. 2009 Koedel U, Frankenberg T, Kirschnek S, Obermaier B, Häcker H, Paul R, Häcker G (2009). Apoptosis is essential for neutrophil functional shutdown and determines tissue damage in experimental pneumococcal meningitis. PLoS Pathog, 5(5), e1000461. 2008 Wang H, Matsuzawa A, Brown SA, Zhou J, Guy CS, Tseng PH, Forbes K, Nicholson TP, Sheppard PW, Häcker H, Karin M, Vignali DA (2008). Analysis of nondegradative protein ubiquitylation with a monoclonal antibody specific for lysine-63-linked polyubiquitin. Proc Natl Acad Sci U S A, 105(51), 20197-202. Into T, Inomata M, Nakashima M, Shibata K, Häcker H, Matsushita K (2008). Regulation of MyD88-dependent signaling events by S nitrosylation retards toll-like receptor signal transduction and initiation of acute-phase immune responses. Mol Cell Biol, 28(4), 1338-47. Frankenberg T, Kirschnek S, Häcker H, Häcker G (2008). Phagocytosis-induced apoptosis of macrophages is linked to uptake, killing and degradation of bacteria. Eur J Immunol, 38(1), 204-15. 2007 El Kasmi KC, Smith AM, Williams L, Neale G, Panopoulos AD, Watowich SS, Häcker H, Foxwell BM, Murray PJ (2007). Cutting edge: A transcriptional repressor and corepressor induced by the STAT3-regulated anti-inflammatory signaling pathway. J Immunol, 179(11), 7215-9. 2006 Wang GG, Calvo KR, Pasillas MP, Sykes DB, Häcker H, Kamps MP (2006). Quantitative production of macrophages or neutrophils ex vivo using conditional Hoxb8. Nat Methods, 3(4), 287-93. Häcker H, Redecke V, Blagoev B, Kratchmarova I, Hsu LC, Wang GG, Kamps MP, Raz E, Wagner H, Häcker G, Mann M, Karin M (2006). Specificity in Toll-like receptor signalling through distinct effector functions of TRAF3 and TRAF6. Nature, 439(7073), 204-7. before 2006 Kirschnek S, Ying S, Fischer SF, Häcker H, Villunger A, Hochrein H, Häcker G (2005). Phagocytosis-induced apoptosis in macrophages is mediated by up-regulation and activation of the Bcl-2 homology domain 3-only protein Bim. J Immunol, 174(2), 671-9. Amir RE, Häcker H, Karin M, Ciechanover A (2004). Mechanism of processing of the NF-kappa B2 p100 precursor: identification of the specific polyubiquitin chain-anchoring lysine residue and analysis of the role of NEDD8-modification on the SCF(beta-TrCP) ubiquitin ligase. Oncogene, 23 (14), 2540-7. Redecke V, Häcker H, Datta SK, Fermin A, Pitha PM, Broide DH, Raz E (2004). Cutting edge: activation of Toll-like receptor 2 induces a Th2 immune response and promotes experimental asthma. J Immunol, 172(5), 2739-43. Seegmuller I, Häcker H, Wagner H (2003). IL-4 regulates IL-12 p40 expression post-transcriptionally as well as via a promoter-based mechanism. Eur J Immunol, 33(2), 428-33. Häcker H, Furmann C, Wagner H, Häcker G (2002). Caspase-9/-3 activation and apoptosis are induced in mouse macrophages upon ingestion and digestion of Escherichia coli bacteria. J Immunol , 169(6), 3172-9. Ahmad-Nejad P, Häcker H, Rutz M, Bauer S, Vabulas RM, Wagner H (2002). Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol, 32(7), 1958-68. Vabulas RM, Ahmad-Nejad P, da Costa C, Miethke T, Kirschning CJ, Häcker H, Wagner H (2001). Endocytosed HSP60s use toll-like receptor 2 (TLR2) and TLR4 to activate the toll/interleukin-1 receptor signaling pathway in innate immune cells. J Biol Chem, 276(33), 31332-9. Bauer S, Kirschning CJ, Häcker H, Redecke V, Hausmann S, Akira S, Wagner H, Lipford GB (2001). Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. Proc Natl Acad Sci U S A, 98(16), 9237-42. Häcker H, Vabulas RM, Takeuchi O, Hoshino K, Akira S, Wagner H (2000). Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumor necrosis factor receptor-associated factor (TRAF)6. J Exp Med, 192(4), 595-600. Vabulas RM, Pircher H, Lipford GB, Häcker H, Wagner H (2000). CpG-DNA activates in vivo T cell epitope presenting dendritic cells to trigger protective antiviral cytotoxic T cell responses. J Immunol, 164(5), 2372-8. Schrezenmeier H, Hildebrand A, Rojewski M, Häcker H, Heimpel H, Raghavachar A (2000). Paroxysmal nocturnal haemoglobinuria: a replacement of haematopoietic tissue? Acta Haematol, 103(1), 41-8. Häcker H, Mischak H, Hacker G, Eser S, Prenzel N, Ullrich A, Wagner H (1999). Cell type-specific activation of mitogen-activated protein kinases by CpG-DNA controls interleukin-12 release from antigen-presenting cells. EMBO J, 18(24), 6973-82. Häcker H, Mischak H, Miethke T, Liptay S, Schmid R, Sparwasser T, Heeg K, Lipford GB, Wagner H (1998). CpG-DNA-specific activation of antigen-presenting cells requires stress kinase activity and is preceded by non-specific endocytosis and endosomal maturation. EMBO J, 17(21), 6230-40. Sparwasser T, Miethke T, Lipford G, Erdmann A, Häcker H, Heeg K, Wagner H (1997). Macrophages sense pathogens via DNA motifs: induction of tumor necrosis factor-alpha-mediated shock. Eur J Immunol, 27(7), 1671-9. Sparwasser T, Miethke T, Lipford G, Borschert K, Häcker H, Heeg K, Wagner H (1997). Bacterial DNA causes septic shock. Nature, 386(6623), 336-7. del Val M, Hengel H, Häcker H, Hartlaub U, Ruppert T, Lucin P, Koszinowski UH (1992). Cytomegalovirus prevents antigen presentation by blocking the transport of peptide-loaded major histocompatibility complex class I molecules into the medial-Golgi compartment. J Exp Med, 176 (3), 729-38. Review Articles Review articles Häcker H, Tseng PH, Karin M (2011). Expanding TRAF function: TRAF3 as a tri-faced immune regulator. [Review]. Nat Rev Immunol, 11, (7), 457-68. Häcker H, Karin M (2006). Regulation and function of IKK and IKK-related kinases. [Review]. Sci STKE, 2006, (357), re13. Häcker H, Karin M (2002). Is NF-kappaB2/p100 a direct activator of programmed cell death? [Review]. Cancer Cell, 2, (6), 431-3. Häcker G, Redecke V, Häcker H (2002). Activation of the immune system by bacterial CpG-DNA. [Review]. Immunology, 105, (3), 245-51. Häcker H (2000). Signal transduction pathways activated by CpG-DNA. [Review]. Curr Top Microbiol Immunol, 247, 77-92. Wagner H, Lipford GB, Häcker H (2000). The role of immunostimulatory CpG-DNA in septic shock. [Review]. Springer Semin Immunopathol, 22, (1-2), 167-71. Wagner H, Häcker H, Lipford GB (2000). Immunostimulatory DNA sequences help to eradicate intracellular pathogens. [Review]. Springer Semin Immunopathol, 22, (1-2), 147-52. Heeg K, Sparwasser T, Lipford GB, Häcker H, Zimmermann S, Wagner H (1998). Bacterial DNA as an evolutionary conserved ligand signalling danger of infection to immune cells. [Review]. Eur J Clin Microbiol Infect Dis, 17, (7), 464-9. Patents Patents/ Patent Applications 2015 Häcker H. Method F or Generation Of Conditionally Immortalized Hematopoietic Progenitor Cell Lines With Multiple Lineage Potential. 2019 Häcker H, Katzenellenbogen J, Pollock J. Toll-Like Receptor Signaling Inhibitors 2022 Haecker H, Redecke V. Rapid Assay For Detection of SARS-CoV-2 Antibodies, US11175293B1 2023 Cahoon B, Smith R, Swenson S, Redecke V, Haecker H, Astill M, Wendling R. Image Analysis For Classifying Hemagglutination And Quantifying Presence Of Antibodies Redecke V, Haecker H, Astill M, Wendling R. Compositions, Devices, and Methods For Performing Agglutination Assays

Explore our past research lab members' accomplishments and see what they are up to today. Alumni. Alumni hold a special place in the heart of our lab, Their diverse journeys post-lab demonstrate the versatility of skills and knowledge they acquired during their time with us. We are proud of our lab alumni who, as future researchers, continue to explore and discover. Daniel J. (DJ) Doty, BS Sr. Laboratory Specialist 2020- 2023 Current Position: Sr. Laboratory Specialist, University of Utah Feroz Ahmed Shaik, PhD Postdoctoral Fellow 2020-2023 Research Emphasis: Identifying Small Molecules Inhibiting Toll-like Receptor (TLR) Mediated Signaling and Inflammation. Current Position: Scientist at Popvax Shaheer Ahmad, PhD Postdoctoral Fellow 2020-2021 Research Emphasis: Role of TNIP1 in SLE Current Position: Postdoctoral Fellow at La Jolla Institute for Immunology Peter Daniels Undergraduate Student 2017- 2018 Research Emphasis: TNIP1 signaling and apoptosis Current Position: Medical Student at VCU School of Medicine Jeeba (Kuriakose) Bellot, PhD Postdoctoral Fellow 2011-2017 Research Emphasis: Role of patrolling monocytes in SLE Publication: Patrolling monocytes promote the pathogenesis of early lupus-like glomerulonephritis A phospho-tyrosine-based signaling module using SPOP, CSK, and LYN controls TLR-induced IRF activity SHARPIN controls the development of regulatory T cells Current Position: Associate Director Flow Cytometry and QC Assay Development at Immatics Sirish Ippagunta, PhD Postdoctoral Fellow 2010-2015 Research Emphasis: TLR drug development, cellular pathogenesis of psoriasis Publication: Identification of Toll-like receptor signaling inhibitors based on selective activation of hierarchically acting signaling proteins Triaryl Pyrazole Toll-Like Receptor Signaling Inhibitors: Structure-Activity Relationships Governing Pan- and Selective Signaling Inhibitors Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function Patrolling monocytes promote the pathogenesis of early lupus-like glomerulonephritis Current Position: Associate Professor at AIIMS (All India Institute of Medical Sciences, New Delhi) Kazuki Tawaratsumida, PhD Postdoctoral Fellow 2010-2016 Research Emphasis: Molecular mechanisms of signal transduction, Influenza host- pathogen interaction Publication: Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor. Identification of Toll-like receptor signaling inhibitors based on selective activation of hierarchically acting signaling proteins A phospho-tyrosine-based signaling module using SPOP, CSK, and LYN controls TLR-induced IRF activity Current Position: Research Associate Haecker Lab Vandana Chaturvedi, PhD Postdoctoral Fellow 2010-2013 Research Emphasis: Role of Sharpin in T-cell differentiation Publication: SHARPIN controls the development of regulatory T cells. Hematopoietic progenitor cell lines with myeloid and lymphoid potential Current Position: Scientist at Sana Biotechnology, Seattle Juan P. Giraldo-Vela, PhD Postdoctoral Fellow 2010-2012 (Redecke Lab) Research Emphasis: Identification of novel components of the T-cell receptor signaling pathway Publication: The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-κB activation. Current Position: Infection Prevention Surveillance Analyst at Advocate Aurora Health David Achila, PhD Postdoctoral Fellow 2013-2015 Research Emphasis: TNIP1 signaling and apoptosis Current Position: Manager, Quality Control Operations, CAR-T Manufacturing Cell Therapy at Bristol-Myers Squibb Rohan Keshwara, PhD, CMPP Undergraduate Student 2009 (Redecke Lab) Research Emphasis: Identify novel components of the T-cell receptor (TCR) signaling pathway Current Position: Senior Manager, Worldwide Publications (Hematology/Cell Therapy) at Bristol Myers Squibb Cinthia Stempin, PhD Postdoctoral Fellow 2008-2010 (Redecke Lab) Research Emphasis: Identification of novel components of the T-cell receptor signaling pathway Publication: The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-κB activation. Current Position: Senior Researcher CONICET Centre for Research in Clinical Biochemistry and Immunology (CIBICI) Cordoba, Argentina Liying Chi, MD Sr Research Technologist 2008-2013 (Redecke Lab) Publication: The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-κB activation. Type I interferon protects against pneumococcal invasive disease by inhibiting bacterial transmigration across the lung. NIK prevents the development of hypereosinophilic syndrome-like disease in mice independent of IKKα activation. Current Position: Sr Research Technologist St Jude Children’s Research Hospital, Memphis Ruiqiong Wu, PhD Associate Scientist 2008-2019 Research Emphasis: Identification of novel components of the T-cell receptor signaling pathway Publication: A phospho-tyrosine-based signaling module using SPOP, CSK, and LYN controls TLR-induced IRF activity. Patrolling monocytes promote the pathogenesis of early lupus-like glomerulonephritis. Hematopoietic progenitor cell lines with myeloid and lymphoid potential. A20-binding inhibitor of NF-kappaB (ABIN1) controls Toll-like receptor-mediated CCAAT/enhancer-binding protein beta activation and protects from inflammatory disease Current Position: Associate Scientist St Jude Children’s Research Hospital Van Phan Undergraduate Student 2009- 2013 Research Emphasis: Molecular mechanisms of nfluenza host- pathogen interaction Publication: Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor. Current Position: Medical Student Kim LeMessurier, PhD Postdoctoral Fellow 2007-2011 (Redecke Lab) Research Emphasis: Host-pathogen interaction in Pneumoccal Disease Publication: Type I interferon protects against pneumococcal invasive disease by inhibiting bacterial transmigration across the lung. Inhibition of T cells provides protection against early invasive pneumococcal disease. Current Position: Lead Scientist at Children's GMP LLC/St. Jude Children's Research Hospital Jennifer Hobbs, PhD Postdoctoral Fellow 2007-2010 Research Emphasis: Signaling via TRAF family members Current Position: Senior Vice President for Administration, Chief of Staff at The New School, New York Jingran Zhou, MD, PhD Postdoctoral Fellow 2006-2012 Research Emphasis: Deciphering the function of TNIP1 (ABIN1) Publication: A20-binding inhibitor of NF-kappaB (ABIN1) controls Toll-like receptor-mediated CCAAT/enhancer-binding protein beta activation and protects from inflammatory disease Patrolling monocytes promote the pathogenesis of early lupus-like glomerulonephritis Sadly, Jingran passed away. She touched all of our lives profoundly and we miss her dearly. Rafijul Bari, PhD Postdoctoral Fellow 2006-2007 (Redecke Lab) Research Emphasis: CD28 receptor signaling Current Position: Director, Research and Early DevelopmentDirector, Research and Early Development Celularity Inc.

We have developed a novel method to immortalize hematopoietic progenitor cells allowing us to gain unique insights into blood cell development under normal and pathological conditions. Generation of conditionally immortalized hematopoietic progenitors BACKGROUND & KEY OBSERVATIONS Hox genes are developmentally important transcription factors. Constitutively active Hox-mutants are oncogenes. Estrogen-regulated Hox-genes can be used to block cell differentiation. The combination of regulated Hox-gene and specific growth factor can be used to immortalize specific progenitor cells with neutrophil potential (Hoxb8-SCF (SCF)) or multi-lineage potential (Hoxb8-FL, FLT3L) (see publication below). CURRENT PROJECTS Conditional immortalization of an erythrocyte/ megakaryocate progenitor. Application of Hox-cells in various models, including inflammatory and infectious disease models to study cell differentiation and contribution of specific immune cell populations to immunity. KEY PUBLICATIONS Wang, Nature Methods, 2006 (PMID: 16554834) Redecke, Nature Methods, 2013 (PMID: 23749299)

Identifying novel components of Toll-like receptor signaling pathways by using cutting-edge proteomic techniques and in vitro and in vivo analysis. Toll-like receptor (TLR) signaling BACKGROUND TLRs represent a family of receptors essential for recognizing pathogens. They initiate signaling via adaptor proteins and partially defined pathways. TLRs upregulate inflammatory genes to initiate immune responses, however, exaggerated/ prolonged TLR activation can lead to inflammatory pathology. OUR MAJOR OBSERVATIONS We identified/ characterized various components of these pathways, including MyD88 and TRAF6 as part of the TLR9 pathway (recognizing DNA), TRAF3 as regulator of interferons and IL-10, and ABIN1/TNIP1 as regulator of the C/EBPb pathway. PROJECT LAYOUT We use primarily artificial dimerization of adaptor proteins and affinity purification to characterize transiently assembled signaling complexes by quantitative mass spectrometry. We characterize identified proteins functionally in vitro and in vivo. CURRENT FOCUS We explore the molecular mechanism of TNIP1 function, which is essential to protect us from inflammatory diseases. We also work on a new ‘signaling protein, which controls the transcription factor family‚ interferon regulatory factors (IRF). KEY PUBLICATIONS Haecker, Nature 2006 (PMID: 16306937) Zhou, PNAS, 2011 (PMID: 22011580) Kuriakose, JCI, 2019 (PMID: 31033479) Tawaratsumida, Sci Adv, 2022 (PMID: 35857476)

Explore the lighter side of science with our just-for-fun website. Just for Fun In our lab, there's always room for laughter and unexpected discoveries, We believe that adding a bit of fun to science can make work and the learning experience truly more enjoyable and captivating.

Transforming healthcare through AI-driven diagnostics. Explore our innovative solutions for accurate and rapid point-of-care testing. Diagnostic Test Development BACKGROUND Antibodies, which are typically generated during immune responses against pathogens, provide important information related to previous infections and, dependent on the specific pathogen and antibody titer, allow conclusions regarding the protection from future infections. Diagnostic tests for antibodies are conducted either in specialized diagnostic laboratories (reference labs) or in point-of-care (POC) settings, with different advantages and disadvantages. Tests performed in reference labs excel in qualitative and quantitative performance and high-throughput capabilities, but have long turn-around times and are expensive. POC tests typically provide quick answers and are cheap, but are often not quantitative and subjective in their interpretation. In this project, we developed an antibody diagnostics platform with (initial) focus on the identification of SARS-CoV2-specific antibodies. OUR MAJOR OBSERVATIONS We found that engineered, bi-specific protein molecules can be used to trigger agglutination in the presence of SARS-CoV2-specific antibodies (AB). These proteins consist (i) in a nanobody (small camelid AB) that binds red blood cells (RBC) and (ii) in part of the virus, e.g. the receptor-binding domain of the Spike protein (Spike-RBD) of SARS-CoV2 (serving as AB target). When added to finger-stick blood, this protein binds immediately RBC. If AB are present, these AB will bind Spike-RBD and lead to visually apparent agglutination. CURRENT PROJECTS We are currently expanding the repertoire of AB tests to other pathogens, and we co-founded a company (Nanospot.AI ) with the goal to commercialize this technology. KEY PUBLICATIONS Redecke, Sci Rep, 2021 (PMID: 34969960)

Here are some handy lab tips and tricks to make your life in the lab easier. Lab-related tricks, tips, and hacks We are always willing to learn! Please let us know if you have any lab wisdom to share! General ALWAYS Label everything! EVERY experiment needs a positive and negative control. Always proofread your emails. Write down every little detail, even if you think you will remember, and you don't think you need to, Vortex/ mix everything before pipetting. Spin down lyophilized reagents before reconstituting. Clean your bench at the end of the experiment to ensure that everything is stored away properly. Ask questions, no matter how stupid or afraid you feel. It is okay to say "I don't know"! Don't throw out essential items (eg. leftover cells or flow through/fractions of purifications) until the end of the experiment, you might still need them if you mess up. Get into the habit of making sure that liquid nitrogen tanks, fridge and freezer doors are closed after you have used them. Label every reagent with the date received or prepared. That way you do not have to guess whether the reagent is still good to use. Plan your experiment and make sure to make your calculations in advance. Always close tubes when disposing of them. Many experiments have been rescued from the trash. Accept that failure is a part of science. Don't be hard on yourself, we have all messed up! Just learn from it! Don't aim to pipette faster, just more accurately. Look at your pipette tip to make sure that you have drawn up the right amount of fluid. Don't be afraid of messing up or bad results. Be honest with your data. Only then you can troubleshoot, and often some data can be salvaged. Everybody makes mistakes and remember, unexpected results can sometime lead to unexpected findings. Always pack snacks! Lab Techniques When using tape for labeling, fold over 1/4 inch on one end. That "handle" will make it so much easier to remove the label. If you need to transfer coverslips, it is easier to pick them up with a Pasteur pipette aspirator instead of forceps. Less breakage and less damage to samples cultured on coverslips! When pipetting solution into several tubes, move your tubes to a different row of your rack once you have added the solution. That way you do not accidentally leave out tubes or add double the amount if you get interrupted . P10, 20 and 200 tips fit onto the end of Pasteur pipettes. That way, you can quickly swap tips when vacuuming solutions if there is a risk of cross-contamination. When transforming ampicillin resistant plasmids or ligations, you can skip the the 30-60 minute incubation and plate the mix out directly onto the bacterial plate. This will not work for kanamycin resistant plasmids. Dry ice mixed with methanol can be used for snap freezing samples instead of liquid nitrogen. When preparing agarose gels microwave the Agarose with only half of the TAE buffer until it is dissolved. Then add the remaining TAE buffer. No more waiting for the agarose to cool down! When pouring stirred solutions, hold a larger magnetic stirrer against the glass while pouring the solution to hold the stir bar back. For solutions that are viscous and difficult/ inaccurate to pipette, eg. glycerol, Tween, prepare a 10% stock solution by weight (1ml = 1g) that you can use for easier pipetting. Write dilutions on the bottle of your stock solutions. That way, you do not need to calculate every time you use the stock. When counting colonies on bacterial plates, mark the colony spots on the outside of the plate with a sharpie, so you do not lose count. Use your tube rack like a washboard to break up stubborn pellets. Do not fill tubes to the rim. Leave 10% space for mixing or for the fluid to expand when freezing. When spinning down small amounts of cells, DNA, protein or other material that is hard to see place the Eppendorf tubes into the centrifuge with the hinges of the tubes facing outwards. That way you will always know where the pellet is even if you do not see it. Keeping Organized I ndex your plasmids, primers, cell lines and your -80. It makes it so much easier to find reagents. In your notebook, put your experiment into context. In a year you will not remember why you did a specific experiment. For each experiment include: Background and scientific question - why are you doing this experiment Materials - list all materials used, include batch/ lot number Experimental Layout - include a detailed protocol for the experiment Results - show the data and analysis Conclusion Questions/ Future Experiments Label your files year-month-day (eg. 2022-10-12). That way your files will always show in chronological order. Create template files for procedures/ calculations often used. Keep an Excel file and include name, company, date, product number of everything you order. Take pictures of reagents, procedures, etc. and stick them in your notebook. Often a picture is worth a thousand words! Presentations/ Publications Less is more. Keep your slides and posters simple. One paper, one message. And most importantly, keep your curiosity and excitement and do not forget to have fun!

The Haecker lab has developed an innovative phenotypic screening platform to identify small molecule inhibitors for the treatment of inflammatory diseases. Drug Development of anti-inflammatory agents BACKGROUND TLR-mediated inflammation promotes a plethora of human diseases, including acute bacterial sepsis, chronic inflammatory diseases (e.g. SLE), cancer (e.g. certain lymphomas) and metabolic diseases, e.g. ischemia-reperfusion injury (cardiac infarction, stroke). Despite the obvious clinical need, no drugs are currently clinically available. This is –at least in part- due to the signaling mechanisms involved, which depend largely on protein interactions (highlighted with yellow dots in figure below). Such interactions are inherently difficult for targeted drug developmental approaches. In turn, while phenotypic approaches could be used, those have the disadvantage of the uncertainty of the drug target. To overcome this problem, we developed a novel phenotypic screening platform that relies on artificial activation of signaling proteins that act at different levels of the TLR pathway (green arrows in figure below). This allows us during the drug-screening campaign to focus on compounds that inhibit the TLR-specific signaling level (upstream of TRAF). We provided proof-of-concept for this approach in recent work. OUR MAJOR OBSERVATIONS We used a bioactive compound library and provided proof of concept for described signaling level-specific phenotypic drug screening. Moreover, using this approach we identifed a small molecule TLR signaling inhibitor scaffold (TSI), whose more detailed analysis related to structure activity relationship and mechanism of action demostrated intruiging properties. CURRENT PROJECT S We currently optimize described TSI scaffold, for further preclinical testing in in vivo inflammatory mouse models. We also initiated a more extensive HTS, in collaboration with the St. Jude Children‘s Research Hospital, to identify additional chemotypes for successful long-term drug development. KEY PUBLICATIONS Ippagunta, Science Signaling, 2018 (PMID: 30108181) Pollock, ChemMedChem, 2018 (PMID: 30117269)

Welcome to the Haecker lab, where we use innovative techniques to understand innate immunity, cell differentiation, and inflammatory diseases. home new PERSPECTIVES “It’s a privilege to run a lab working on something I find fascinating and believe has real impact. To work towards the end goal to make a real-world difference, through the highs and lows with a dedicated team, is a joy. And the excitement of finding something new never gets old!” Hans Haecker- Futurum Career WHAT WE DO WELCOME TO OUR LAB! where we are working together on fundamental and translational aspects of Immunology to advance human health and train the next generation of biomedical scientists. The major focus of our lab is on innate immunity and inflammation, with projects ranging from molecular mechanisms of signal transduction to translational aspects of drug development. We explore how innate immune cells recognize and respond to pathogens, how genetic mutations in innate immunity contribute to inflammatory and auto-immune diseases, and how obtained information can be used to develop novel therapeutic strategies. To learn more about us, our educational and research programs, career opportunities and the latest news, please explore our website, connect with us on Twitter , LinkedIn or contact us via email ! Join Us Just for Fun Lab Hacks- Tips and Tricks 01 COLLABORATION 02 INNOVATION 03 MENTORSHIP 04 IMPACT Research OUR RESEARCH Toll-like receptor (TLR) signaling TLRs represent a key family of pathogen recognition receptors that alert the immune system upon pathogen encounter via inflammation. We use proteomic approaches (quantitative mass spectrometry) to identify novel components of TLR signaling pathways, whose function we explore in vitro and in vivo. Our goal? To understand immunity better! more details on our TLR signaling research Inflammatory Diseases We have developed novel mouse models based on human genetic information for the inflammatory diseases systemic lupus erythematosus and psoriasis. We use these models to explore the pathogenic mechanisms involved in order to identify direly needed novel therapeutic targets against these specific, but also more broadly, inflammatory diseases. learn more about our research on inflammation Hematopoiesis We utilize regulated Hox-genes to precisely target and immortalize distinct hematopoietic progenitor cells. These modified cells serve as essential models for studying the intricate processes of cell differentiation and immune effector functions. By conducting experiments both in controlled laboratory environments (in vitro) and living organisms (in vivo), we aim to unravel the complexities underlying these biological phenomena and contribute to a deeper understanding of hematopoiesis and immune responses. more details on our research on hematopoiesis Drug Development We have developed a novel and unique phenotypic screening platform that enables us to discover small molecule compounds with potential therapeutic applications in inflammatory diseases. This innovative platform enables us to methodically evaluate and select compounds that exhibit specific desired effects, thereby elevating our ability to contribute to the development of effective treatment strategies in the field of inflammation-related disorders. learn more about our drug development projects Diagnos tic Test Development We have co-founded a diagnostic company focused on creating advanced point-of-care antibody diagnostics. Our innovative approach combines hemagglutination reactions with artificial intelligence to allow accurate, affordable and rapid diagnostic test right on your smartphone. more details on our diagnostic test developments News NEWS & EVENTS OUR TEAM Hans Haecker MD | PhD Professor Microbiology & Immunology Co-Founder NanoSpot.ai I've always had a love for sports, but unfortunately, that often comes with its fair share of injuries. Those frequent trips to orthopedic surgeons from a young age sparked my interest in becoming a medical doctor, especially in the field of sports medicine. My fascination with sports medicine never waned, but I felt that my medical work could benefit from a stronger foundation in science. This led me to pursue a PhD and postdoctoral training after medical school, and I ended up delving deeper into research. Still, my focus remains on making research findings practical in the field of medicine, ensuring that what we discover benefits patients directly. MD University of Ulm, Germany PhD Technical University Munich, Germany Postdoc University of California, San Diego Assistant/ Associate Member St. Jude Children’s Research Hospital, Memphis Email: hans.haecker@path.utah.edu Phone: 801-587-1507 Hans Haecker Publication List Vanessa Redecke, MD, PhD Assistant Research Professor Co-Founder NanoSpot.ai MD University of Lubeck, Germany PhD University of Lubeck, Germany Postdoc University of California, San Diego Assistant Member/ Staff Scientist St. Jude Children’s Research Hospital, Memphis Email: v anessa.redecke@path.utah.edu Phone: 801-213-8507 Kazuki Tawaratsumida, PhD Research Associate Research Emphasis: Molecular mechanisms of signal transduction PhD Kagoshima University, Japan Postdoc St Jude Children’s Research Hospital, Memphis Email: kazuki.tawaratsumida@path.utah.edu Phone: 801-585-6711 Ravi Shankar Singh, PhD Postdoctoral Research Associate Research Emphasis: Molecular and cellular mechanisms of inflammatory diseases MSc Sardar Patel University, VV Nagar, Gujarat, India PhD Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Lucknow, India Postdoc University of Saskatchewan, Saskatoon, Canada Email: ravi.singh@path.utah.edu Phone: 801-585-6711 Laith F. Al Rabadi, MD Associate Professor (Clinical), Internal Medicine Research Emphasis: Membranous Nephropathy M.B.B.S Jordan University of Science and Technology (Medicine) Postdoc Harvard-MIT Division of Health Sciences and Technology Fellowship Boston Medical Center, Boston University Email: Laith.Al-Rabadi@path.utah.edu Phone: 801-581-6709 Faraz Salehi, MS Graduate Student Research Emphasis: Hematopoiesis directed by multi-potent progenitor cells MS Shahid Beheshti University, Tehran, Iran Email: faraz.salehi@path.utah.edu Phone: 801-585-6711 Lucia Ranallo. BS Master Student/ Research Technician Current Sudies : Master in Biomedical Informatics from the University of Utah BS St. Olaf College, Minnesota Malak Anees (Angel) Alsammarraie Undergraduate Student Research Emphasis: Molecular mechanism of TLR signaling University of Utah (Major: Biology) Phone: 801-585-6711 Alumni JOIN US We are always looking to expand our collaborative research team. Become a member of our lab, where you will have access to state-of-the-art facilities, mentorship and a supportive and intellectually stimulating environment. Please feel free to contact us for additional information. To apply, please send • a cover letter containing previous research experiences and specific interests in the Haecker lab • a CV containing a complete list of publications, • the addresses of three professional references to hans.haecker@path.utah.edu . Postdoctoral Fellows Are you interested in immunology, inflammation biology, cell signaling or drug development? We offer plenty of training opportunities, mentorship and great work-life balance for postdocs. Requirements are a recent MD or PhD in molecular biology, immunology, biochemistry or other life science related topic. Graduate Students Graduate school is where you dive deeper into what you love, and acquire specialized knowledge and tools that will help you get ready for your future job. We offer personalized mentorship, extensive training and a supportive environment that will empower you to make a meaningful impact and help you get ready for your future job. You can either apply for a PhD program through the M&I Graduate Program or Bioscience Graduate Program. Interested in a PhD, but unsure if it is right for you? Our lab offers the opportunity to join us for a year as a "research technician". Once you have completed that year and if we are both satisfied and agree, you will be able to directly enroll into our M&I Graduate program as a graduate student in our lab. Please contact us for more information. Undergraduate Students As an undergraduate research student in our lab, you will step into the world of research and bring your learning to life with hands-on training. You will collaborate with experienced mentors, delve into meaningful projects, and see your ideas make a difference.. ResearchTechnicians Become a part of our research team, where you'll work alongside experienced scientists, use cutting-edge technologies, and use your skills to make a difference. Your role will play a crucial part in advancing knowledge and pushing the boundaries of what we know. We are looking for research technician at all levels with particular interest/ expertise in immunology, molecular biology and/or mouse models of autoimmunity and/or inflammatory diseases. Requirements are a Bachelor’s degree in Biology, Chemistry, or other scientific discipline. Join Us Teaching TEACHING Supporting your growth through mentoring and teaching is a priority in our lab. Feel free to visit us anytime – our door is open for any questions you may have. Your development matters to us, and we're here to assist you every step of the way. Hans Haecker is director of the Basic Immunology courses (PATH7330 & PATH5030) offered every Fall, teaches part of the Topics in Immunology (Path 7320) course as well as the Capstone course. Hans is also part of the Molecular Biology Program - Innate Immunity and Inflammation and currently mentors 14 PhD/Doctorate students outside the lab on dissertation committees. Collaborations COLLABORATIONS We're all about partnering up – whether it's among our lab members or extending our connections to the wider world. If you're someone who enjoys collaborating, you'll find a home with us! TLR signaling and inflammation Dr. Patrick Walker, CEO Arkana Laboratories, Little Rock (kidney pathology in lupus patients) Dr. Peter Vogel, Director Veterinary Pathology Core, Department of Pathology, St. Jude Dr. Heather Tillman, Assistant Professor, Department of Pathology, St. Jude Dr. Marc Barry, Associate Professor, Department of Pathology, University of Utah Dr. James Cox, Director Mass Spectrometry & Proteomics, University of Utah Dr. Cliff Guy, Managing Director, Imaging Facility Department of Immunology, St.Jude TLR drug development Dr. Taosheng Cheng, Director High through put screening (HTS) core, St. Jude Dr. John Katzenellenbogen, Professor, University of Illinois, Urbana Dr. Julie Pollock, Assistant Professor, Chemistry Department, University of Richmond Dr. Richard Lee, Member, Department of Chemical Biology and Therapeutics, St. Jude Conditionally immortalized hematopoietic progenitor cells Dr. Thomas Vogl, Professor, Department of Immunology, University of Münster Dr. Barbara Walzog, Professor, Department of Physiology, LMU Munich Dr. Uwe Koedel, Professor, Department of exp. Neurology, LMU Munich Dr. Michael Sixt, Professor, Institute of Science and Technology, Vienna Dr. Georg Häcker, Director Inst. of Microbiology and Hygiene, University of Freiburg Dr. Boris Reizis, Professor, Department of Pathology, New York University Dr. Stephen Nutt, Professor, WEHI institute, Melbourne Dr. Thomas Graf, Senior Scientist, Centre for Genomic Regulation, Barcelona Dr. Philippe PIERRE, Professor, Centre d’Immunologie de Marseille-Luminy Dr. Peter VanLent, Professor, Radboud University Medical Center, Nijmegen Dr. Mortimer Poncz, Professor, University of Pennsylvania Dr. Tobias Hohl, Chief, Infectious Diseases Service, Memorial Sloan Kettering, New York Useful Links Scientific Resources USEFUL SCIENTIFIC LINKS Here are some useful scientific link that we often use in our lab. We hope that they will help you find answers to your questions! BASIC SCIENTIFIC TOOLS PubMed NCBI BLAST Molarity Calculator Fluffyfrog's Serial Dilution Calculator Primer3 Primer design CLONING & GENE EXPRESSION NEBuffer Activity/Performance Chart with Restriction Enzymes NEBcutter Takara web-based sgRNA design tools Entrez Gene Addgene Reverse Complement DNA sequence PROTEINS & PROTEOMICS ProtParam - physical and chemical parameters for a given protein Protein domain prediction Alphafold - Protein structure prediction String protein interaction database IntAct protein interaction database The Comprehensive Resource of Mammalian Protein Complex (CORUM) UniProt InterPro functional analysis of proteins PhosoSitePlus protein post-translational modification tool Expasy protein modification databases NCBI protein research tools and databases PAXdb: Protein Abundance Database The Human Protein Atlas (human protein expression in cells, tissues and organs) NCBI Protein Domains and Macromolecular Structures SMART domain architecture tool Proteopedia 3D-encyclopedia of proteins & other biomolecules GENOMIC RESOURCES Haemosphere (search for genes of interest, view their expression profiles across a range of cell types) Bloodspot (gene expression in hematopoietic cells at different maturation stages) Gene expression pattern in tissue GWAS, susceptibility, risk lo cBioPortal (cancer genomic GeneCards: The Human Gene Database Mouse Genome Informatics (MGI) Mouse Phenome Databas I International Mouse Phenotyping Consortium. UCSC Genome Browser Genotype-Tissue Expression (GTEx) Gene Set Enrichment Analysis (GSEA) for dataset comparison DRUG DEVELOPMENT Pubchem FDA Drug Approvals and Databases Snip Web (for conversion of chemical structure images into SMILES) IMAGING Histology Guide human histology slides The Cell Image Library repository PATHWAY ANALYSIS & BIOINFORMATICS DAVID pathway analysis g:Profiler pathway analysis KEGG pathway analysis Reactome pathway analysis Cytoscape pathway visualizer IMMUNOLOGY Immunology at Work Resource Center Current Protocols in Immunology Cold Spring Harbor Protocols Immunopaedia - Clinical Cases, Immunology Learning, and Treatment and Diagnostics The Antibody Resource Page Chromocyte flow cytometry resource FACTS & FUN Bionumbers database of useful biological numbers Cell Biology by the Numbers MIT Technology Review Newsletter PhD comics OTHERS ClinicalTrials.gov US Patent and Trademark Office (PTO) WIPO International Patent Applications Heatmapper data visualizer Biorender Wordhippo Thesaurus and word tool Wordtune AI-based writing companion University Resources UNIVERSITY RESOURCES The University of Utah provides access to cutting-edge facilities, expert mentors, and extensive libraries, that will allow you to pursue your interests and successfully complete your research. Experience a campus environment that supports your academic career and fosters innovative technologies, collaborations, and a vibrant community of researchers. GETTING AROUND Campus Map University of Utah shuttle live tracker U niversity of Utah Commuter Services DEPARTMENTS & INITIATIVES Division of Microbiology & Immunology Department of Pathology Immunology, Inflammation, & Infectious Disease (3i) Initiative Environmental Health and Safety Pathology Newsletter IT SERVICES & LOGIN Path-IT support SendIt application for large file transfer Campus IT Help Desk University of Utah CIS login University of Utah Pulse login CORE FACILITIES HSC Core Facilities University of Utah Core Lab Scheduler STUDENT & POSTDOC RESOURCES Microbiology & Immunology PhD program Bioscience PhD program MD-PhD program International Student & Scholar Services Office of Postdoctoral Affairs University of Utah Postdoctoral Association Training Grants and Initiatives E-tutoring and the online writing lab Housing FOOD & RECREATION Campus Recreation Services Utah Athletics University of Utah Dining Services CALENDARS Bioscience Calendar Pathology Department Calendar University of Utah Academic Calendar University of Utah Holiday Calendar Campus Events Calendar Life in Utah LIFE AT THE U AND BEYOND Life in Utah offers a unique blend of natural beauty, outdoor activities, cultural activities and a strong sense of community. With its stunning landscapes, including national parks like Zion, Bryce Canyon, and Arches, outdoor enthusiasts find ample opportunities for hiking, camping, skiing, mountain biking and exploring. Utah also boasts a growing cultural scene, with Salt Lake City being a hub for arts, music, and diverse cuisine. Utah's friendly atmosphere contribute to a welcoming environment, making it a place where people can enjoy a balanced lifestyle. Life amidst the mountains and deserts will offer you a unique backdrop for your academic journey!. WHY UTAH & LIFE AT THE U Ten Reasons to do Graduate Studies in Utah The 15 Best Activities for College Students in Utah! Life at the U Living in Utah Why Utah Campus Recreation Student Affairs Graduate Students Child Center & Family Resources Student Health Center MOVING TO UTAH Moving to Utah- Quick Reference for Utah Newcomers University Housing Finding Housing in Utah Rentler- Apartments and Houses for Rent Apartments for Rent Hotpads- Apartments and Houses for Rent THINGS TO DO IN SALT LAKE Salt Lake Magazine guide to the best restaurants, bars, events, outdoor fun, culture and more. Visit Salt Lake Gastronomic SLC Salt Lake Tribune Calendar Salt Lake City Weekly Salt Lake City Kid out and about Best Trails and Hikes in Salt Lake City THINGS TO DO IN UTAH Utah Vacation Planning and Things to Do Utah Travel and Visitor Information Utah Stories Now Playing Utah Best Trails and Hikes in Utah Ski Utah connect CONNECT WITH US And we promise that you do not need to use a message in a bottle! Just email us at hans.haecker@path.utah.edu . We are located in room 1520 on the first floor of the Emma Eccles Jones Medical Research Building on the upper campus of the University of Utah. For information on public transportation and parking options please see the commuter services website If you have questions finding us, you can always contact us or call the main office at 801-581-4390. Haecker Lab Division of Microbiology & Immunology Department of Pathology University of Utah Emma Eccles Jones Medical Research Building, Rm 1520 H 15 N. Medical Drive East Salt Lake City, UT 84112 hans.haecker@path.utah.edu | +1 801-587-1507 Collaborate, experiment, thrive - Find your place in our dynamic research team. Join us and unlock your potential today!

The Haecker Lab specializes in investigating molecular mechanisms of inflammatory diseases, with the goal of identifying new approaches for treatment. Inflammatory Diseases BACKGROUND We indentified ABIN1/TNIP1 as component of the TLR signaling complex, which is essential to counteract pro-inflammatory TLR signaling. Human TNIP1 is genetically linked to the inflammatory diseases “systemic lupus erythematosus (SLE)“ and “psoriasis“ (hypomorphic polymorphisms). SLE is an “autoimmune“ disease of unclear etiology characterized by auto-antibodies and inflammatory infiltration of many organ systems. Immune complex-deposits in the kidneys are believed to drive glomerulonephritis leading to kidney failure. Psoriasis is an chronic, inflammatory skin disease, characterized by proliferating epidermal cells (keratinocytes), leading to red, scaling patches. OUR MAJOR OBSERVATIONS TNIP1-/- mice develop constitutively the major symptoms of human SLE and, inducibly, psoriasis-like disease. Consistent with current models, SLE is driven by nucleic acid-recognizing TLRs (TLR7/9, MyD88), while psoriasis is driven by the IL-17R. Surprisingly, genetic deletion of T- and B-cells (and thus IgG) did not provide protection from kidney disease. In contrast, we found that a monocyte subtype (Patrolling monocytes, Pmo), accumulates in kidney glomeruli and, intruigingly, that genetic deletion of PMo protected from GN. As such, innate immune cells, not B-cell-derived auto-antibodies promote GN, suggesting a shift in paradigm with important ramnifications for human disease and therapeutic approaches. CURRENT PROJECTS We explore the mechanism of patrolling monocyte (PMo) deregulation. We investigate why PMo are up-regulated (cell differentiation vs. survival) and study the contribution of C/EBPb to PMo deregulation and disease. We also investigate established and novel therapeutic strategies to prevent PMo- mediated disease. KEY PUBLICATIONS Zhou, PNAS, 2011 (PMID: 22011580) Ippagunta, PNAS, 2016 (PMID: 27671649) Kuriakose, JCI, 2019 (PMID: 31033479)

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